Correlation of DNA ploidy and cell cycle analysis with diffusion tensor and dynamic susceptibility contrast MRI metrics in meningiomas

George A. Alexiou, Anastasia K. Zikou, Georgios Vartholomatos, Anna Goussia, Spyridon Voulgaris, Athanasios P. Kyritsis, Maria I. Argyropoulou

Abstract


Purpose: To investigate the relationship between diffusion tensor imaging and dynamic susceptibility perfusion MRI metrics with cell cycle analysis findings in meningiomas.

Material and Methods: Fourteen patients (4 men, 10 women, aged 30-76 years; median 58 years) with meningiomas were included in the study. There were 9 grade I and 5 grade II meningiomas. The MRI protocol consisted of T2-weighted turbo spin echo, unenhanced and contrast enhanced T1-weighted 3D spoiled gradient echo sequences. Diffusion tensor imaging (DTI) metrics, such as apparent diffusion coefficient (ADC) and fractional anisotropy (FA), were assessed with a single shot, multi-slice, spin-echo planar sequence (max b-value: 700 sec/mm²) and dynamic susceptibility perfusion imaging (DSC) metrics, such as the relative cerebral blood volume (rCBV), with a T2* gradient-echo, multishot echo planar imaging sequence. DNA ploidy and cell cycle analysis was analysed by flow cytometry.

Results: There was a significant negative correlation between rCBV and G0/G1 phase fraction (r=−0.791, p=0.004) and a positive correlation with G2/M phase fraction (r=0.621, p=0.04). A significant correlation was also observed between FA ratio and G0/G1 phase fraction (r=0.721, p=0.018). No significant correlation was found between G0/G1, S-phase, G2/M and ADC values.

Conclusions: DSC MRI and DTI metrics are correlated to meningioma aggressiveness as assessed by cell cycle analysis.


Keywords


MRI; diffusion tensor imaging; perfusion imaging; meningioma; cell cycle

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References


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