A multiscale approach for the study of x-ray radiation effects in paediatric patients subjected to interventional cardiology procedures

Maria Souli, Agapi Ploussi, Ellas Spyratou, Ioannis Seimenis

Abstract


Purpose: Paediatric dosimetry requires a patient-spe- cific approach due to the long-life expectancy and high radiosensitivity of children. The main aim of the current study was to study physical and biological dosimetric characteristics, as well as the effect of x-ray radiation on the biochemical properties of lymphocytes in paediatric patients who underwent interventional cardiology (IC) procedures.

Materials and Methods: A total of 10 paediatric pa- tients who underwent IC procedures with a biplane angi- ographic system was enrolled in the study. Physical do- simetry was performed by converting dose-area-product to effective dose with the use of appropriate k-factors taking into account patient’s body size. Peripheral blood samples were collected from each patient before and im- mediately after the IC procedure. Biodosimetry, for the detection of radiation-induced DNA damage, was based on the assessment of the protein biomarker γ-H2AX. Furthermore, biomechanical properties of unirradiated and irradiated lymphocytes were evaluated using atomic force spectroscopy.

Results: Effective doses (EDs) estimated for the stud- ied cases ranged from 0.6 to 16.7 mSv. Immunofluores- cence microscopy detected a small increase in γ-H2AX


Keywords


paediatric dosimetry, γ-H2AX, biodosimetry, elastic modulus, atomic force spectroscopy

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References


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DOI: http://dx.doi.org/10.36162/hjr.v5i1.335

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